Year: 2014 | Month: July | Volume 7 | Special Issue
Molecular characterization of bacterial leaf blight resistant near isogenic lines of rice (Oryza sativa L.) using RAPD and SSR markers
Abstract:
Rice (Oryza sativa L.) is nutritionally, one of the most important cereal crops. Rice production is mostly reduced due to different biotic stresses. Bacterial leaf blight, caused by Xanthomonas oryzae pv. oryzae bacteria, is one of the major biotic destructive diseases throughout the world. This disease affects the rice production because it reduces the photosynthesis by causing leaf wilting. The preferred strategy for disease management is through varietal resistance because it is not fully controlled by any chemical treatments. Gene pyramiding is the most effective strategy; in which, pyramided lines with three to four different bacterial leaf blight resistance genes have been developed in the genetic background of popular rice cultivars by using marker-assisted selection. The present study was carried out with an aim to study the molecular characterization of 30 rice genotypes for BLB resistance using RAPD and SSR markers. Overall results on the basis of clustering pattern of SSR and RAPD pooled data analysis revealed genetic similarity between some of the pyramided lines with their respective recurrent parent. Some of the SSR markers are very informative and can be useful for marker-assisted selection.
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